Genetic polymorphism

Primer sequence

Annealing temperature

Amplicon size

rs12255372 (G/T)

Forward inner primer (T allele)

5’CTGCCCAGGAATATCCAGGCAAGAGTT 3’

58˚C

362 bp (T allele)

Reverse inner primer (G allele)

5’GAGGCCTGAGTAATTATCAGAATATGATC 3’

281 bp (G allele)

Forward outer primer

5’ GGCTGTATGAAGTCATTTGATGATTGTTT 3’

587 bp (from two outer primers)

Reverse outer primer

5’ACGCTTTGAAGGTAGAGAGGACACACT 3’

rs7903146 (C/T)

Forward inner primer (C allele)

5’CAATTAGAGAGCTAAGCACTTTTTAGAGAC 3’

58˚C

306 bp (C allele)

Reverse inner primer (T allele)

5’TGCCTCATACGGCAATTAAATTATAGAA 3’

172 bp (T allele)

Forward outer primer

5’GTAATGCAGATGTGATGAGATCTCTG 3’

420 bp (from two outer primers)

Reverse outer primer

5’AGAAAAATACAAAGACATGCAAAAGC 3’