Genetic polymorphism | Primer sequence | Annealing temperature | Amplicon size |
rs12255372 (G/T) | Forward inner primer (T allele) 5’CTGCCCAGGAATATCCAGGCAAGAGTT 3’ | 58˚C | 362 bp (T allele) |
Reverse inner primer (G allele) 5’GAGGCCTGAGTAATTATCAGAATATGATC 3’ |
| 281 bp (G allele) | |
Forward outer primer 5’ GGCTGTATGAAGTCATTTGATGATTGTTT 3’ |
| 587 bp (from two outer primers) | |
Reverse outer primer 5’ACGCTTTGAAGGTAGAGAGGACACACT 3’ |
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rs7903146 (C/T) | Forward inner primer (C allele) 5’CAATTAGAGAGCTAAGCACTTTTTAGAGAC 3’ | 58˚C | 306 bp (C allele) |
Reverse inner primer (T allele) 5’TGCCTCATACGGCAATTAAATTATAGAA 3’ |
| 172 bp (T allele) | |
Forward outer primer 5’GTAATGCAGATGTGATGAGATCTCTG 3’ |
| 420 bp (from two outer primers) | |
Reverse outer primer 5’AGAAAAATACAAAGACATGCAAAAGC 3’ |
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