No

Level

Reagent (ml)

Temperature (˚C)

Time (min)

Reagent ratio

1

A1B1C1D1

3.5

40

15

0.25:4.25

2

A1B2C2D2

3.5

50

20

0.50:4.00

3

A1B3C3D3

3.5

60

25

0.75:3.75

4

A2B1C2D3

4.0

40

20

0.75:3.75

5

A2B2C3D1

4.0

50

25

0.25:4.25

6

A2B3C1D2

4.0

60

15

0.50:4.00

7

A3B1C3D2

4.5

40

25

0.50:4.00

8

A3B2C1D3

4.5

50

15

0.75:3.75