No | Level | Reagent (ml) | Temperature (˚C) | Time (min) | Reagent ratio |
1 | A1B1C1D1 | 3.5 | 40 | 15 | 0.25:4.25 |
2 | A1B2C2D2 | 3.5 | 50 | 20 | 0.50:4.00 |
3 | A1B3C3D3 | 3.5 | 60 | 25 | 0.75:3.75 |
4 | A2B1C2D3 | 4.0 | 40 | 20 | 0.75:3.75 |
5 | A2B2C3D1 | 4.0 | 50 | 25 | 0.25:4.25 |
6 | A2B3C1D2 | 4.0 | 60 | 15 | 0.50:4.00 |
7 | A3B1C3D2 | 4.5 | 40 | 25 | 0.50:4.00 |
8 | A3B2C1D3 | 4.5 | 50 | 15 | 0.75:3.75 |