Cell capture structures

Targeted cells

Carrier medium and control cells

Target cell recovery rate

Capture purity

An electrode affinity column with interdigitated micro-electrodes

Human leukemic cells of HL-60

Blood cells + sucrose solution

NA

~80% (initial mixture of 2 × 107 HL-60 and 3 × 107 blood cells)

A dielectric affinity column

with interdigitated micro-electrodes

Human breast cancer cells of MDA-231

Blood samples + sucrose

solution

NA

~95% (initial mixture of 1 × 107 MDA-231 and 3 × 107 blood cells)

A dielectric affinity column

with an interdigitated

micro-electrodes

Human breast cancer cells of MDA-231

Blood samples + sucrose

solution

>95%

NA

A dielectric affinity column

with reconfigurable

electrodes

Human cervical cancer cells of HeLa

Human peripheral blood cells +

sucrose solution

NA

NA

A dielectric affinity column

with a micro-electrode

array

Human monocytic cells of

U937, lymphoma cells of

Jurkat, HTLV-1, tax-transformed human T cells of Ind-2, glioma cells of HTB, and

neuroblastoma cells of SH-SY5Y

Peripheral blood mononuclear

cells + sucrose solution

47% - 79%

>95%

DEP field flow fraction with

interdigitated electrodes

Human leukemic cells of HL-60

WBCs from blood samples +

sucrose solution

NA

NA

DEP field flow fraction with

interdigitated electrodes

Human breast cancer cells of MDA-435

Hematopoietic CD34+ stem

cells + sucrose solution

NA

>99% (initial MDA-435: stem cells = 2:3)

DEP field flow fraction with

interdigitated electrodes

Human breast cancer cells of MDA-435

Blood samples + sucrose solution

NA

>98% of MDA-435 (initialMDA-435: blood cells = 2:3)

DEP field flow fraction with

interdigitated electrodes

Human breast cancer cells of

MDA-435, MDA-468 and

MDA-231

Peripheral blood mononuclear

cells + sucrose solution

>90%

NA

Microscope slides coated

with electrode arrays with

changing frequencies

Human breast cancer cells of

MDA-435 and leukemic cells of HL-60

Blood samples + sucrose

NA

NA

Microscope slides coated

with electrode arrays with

changing frequencies

Cancer cells from biopsy

Biopsied cells + sucrose

solution

NA

NA

3D-asymmetric micro-electrodes with a continuously varied

electric field

Mouse P19 embryonic

carcinoma cells

Mouse RBCs + PBS

NA

81.5% ± 7.6% of P19 EC

and 94.1% ± 4.3% RBCs

(initial ratio 1:1)

3D-asymmetric micro-electrodes with a continuously varied

electric field

Human breast cancer cells of

MCF-7 and MCF-10A

PBS

86.67% of MCF-7 and 98.73% of MCF-10A

NA

DC-dielectrophoresis

Fixed WBCs and human breast

cancer cells of MCF-7

Trehalose solution

NA

NA

Guided DEP with a pair of

planar electrodes

Human leukemic cells of Jurkat

and cervical cancer cells of

HeLa

Sucrose solution

NA

NA

Planar interdigitated

Microelectrodes

Clones of mouse melanoma

B16F10 cells

Sucrose solution

NA

NA

A planar electrode pair with

an angle to the flow

direction

Human colorectal cancer cells

of HCT116 and embryonic

kidney cells of HEK 293

PBS

NA

95% of HCT116