Cell capture structures | Targeted cells | Carrier medium and control cells | Target cell recovery rate | Capture purity |
A nickel micropillar array + magnetic beads functionalized with wheat germ agglutinin | Human lung cancer cells of A549 | Human RBCs + culture medium | 62% - 74% | 93% (initial ratio A549:RBCs 1/4 1:10) |
A paramagnetic array of 80% Ni and 20% Fe+ magnetic beads complementary to anti-CD10 antibodies in chamber 1 and anti-PSMA antibodies in chamber 2 | Human prostate cancer cells of LNCaP incubated with PSMA antibodies | LNCaP incubated with CD10 antibodies + PBS | 50% - 70% of LNCaP incubated with PSMA antibodies in chamber 2 | 10% of LNCaP incubated with CD10 antibodies in chamber 2 (initial mixture ratio of 1:1) |
External permanent magnet + magnetic beads coated with Anti-EpCAM (Ber-EP4) | Human ovarian cancer cells of BG-1 and lung cancer cells of AS2 | Blood samples from healthy donors (106 cells per ml) | 95.1% for BG-1 cells and 92.7% for AS2 cells | NA |
An array of magnetic dots + self-assembled magnetic beads coated with anti-CD19 antibodies | Human lymphoma cells of Raji CCL-86 | Human lymphoma cells of Jurkat TIB152 + PBS (2 × 106 cells per ml) | 97% ± 2% of Raji cells | <2% (capture of Jurkat TIB152 cells) |
An array of magnetic dots + self-assembled magnetic beads coated with anti-CD19 antibodies | B-cell hematological malignant tumors (leukemia and lymphoma) | Clinical samples (blood, pleural effusion, and fine needle aspirates) from chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma and two healthy volunteers | Consistent immunophenotype and morphology results with those obtained by flow cytometry | |
External permanent magnet + self-assembled magnetic bead patterns coated with 5D10 antibodies | Human breast cancer cells of MCF-7 | Human lymphoma cells of Jurkat TIB152 + PBS (106cells per ml for both MCF-7 and Jurkat cells) | 85% ± 10% of MCF-7 | <5% (capture of Jurkat TIB152 cells) |
A nickel micropillar array + magnetic beads functionalized with wheat germ agglutinin | Human lung cancer cells of A549 | PBS | A total mass of 90.6 ng of captured A549 cells | |
External permanent magnet + Fe3O4 magnetic nanoparticles conjugated to Anti-EpCAM | Human colon cancer cells of COLO205 and human breast cancer cells of SK-BR-3 | Blood samples from healthy donors | 90% and 86% for COLO205 and SK-BR-3 cells, respectively | NA |