Strains/plasmids | Genotype/phenotype | Reference |
Strains |
|
|
Z. mobilis ZM4 | Wild type | From ATCC |
E. coli DH5α | Used for cloning | The lab stock |
Plasmids |
|
|
pBBR1MCS2 | The broad-host-range vector, kanR | Kovach et al. (1995) [23] |
pC1S1-3TGG | The spacer 1 of ZM4 CRISPR locus P1 with the PAM (TGG) at 3’-end in pBBR1MCS2 | This study |
pC1S1-3GG | The spacer 1 of ZM4 CRISPR locus P1 with the PAM (GG) at 3’-end in pBBR1MCS2 | This study |
pC1S3-3AGG | The spacer 3 of ZM4 CRISPR locus P1 with the PAM (AGG) at 3’-end in pBBR1MCS2 | This study |
pC1S3-3GG | The spacer 3 of ZM4 CRISPR locus P1 with the PAM (GG) at 3’-end in pBBR1MCS2 | This study |
pCri01 | Derived from pBBR1MCS2 containing the ZM4 CRISPR locus 1 with 5’-and 3’-regions | This study |
pC1Z0293.S1 | In pCri01, the spacer 1 is replaced with a 32-bp target sequence from the ZM4 gene ZMO0293 | This study |
pC1Z0293.S3 | In pCri01, the spacer 3 is replaced with another 32-bp target sequence from the gene ZMO0293 | This study |
pC1Z0293.S1/3 | In pCri01, the spacer 1 and 3 are replaced with the above two 32-bp target sequences from ZMO0293 | This study |
pC1Z0875.S1 | In pCri01, the spacer 1 was replaced with a 32-bp target sequence from the gene ZMO0875 | This study |