Strains/plasmids

Genotype/phenotype

Reference

Strains

Z. mobilis ZM4

Wild type

From ATCC

E. coli DH5α

Used for cloning

The lab stock

Plasmids

pBBR1MCS2

The broad-host-range vector, kanR

Kovach et al. (1995) [23]

pC1S1-3TGG

The spacer 1 of ZM4 CRISPR locus P1 with the PAM (TGG) at 3’-end in pBBR1MCS2

This study

pC1S1-3GG

The spacer 1 of ZM4 CRISPR locus P1 with the PAM (GG) at 3’-end in pBBR1MCS2

This study

pC1S3-3AGG

The spacer 3 of ZM4 CRISPR locus P1 with the PAM (AGG) at 3’-end in pBBR1MCS2

This study

pC1S3-3GG

The spacer 3 of ZM4 CRISPR locus P1 with the PAM (GG) at 3’-end in pBBR1MCS2

This study

pCri01

Derived from pBBR1MCS2 containing the ZM4 CRISPR locus 1 with 5’-and 3’-regions

This study

pC1Z0293.S1

In pCri01, the spacer 1 is replaced with a 32-bp target sequence from the ZM4 gene ZMO0293

This study

pC1Z0293.S3

In pCri01, the spacer 3 is replaced with another 32-bp target sequence from the gene ZMO0293

This study

pC1Z0293.S1/3

In pCri01, the spacer 1 and 3 are replaced with the above two 32-bp target sequences from ZMO0293

This study

pC1Z0875.S1

In pCri01, the spacer 1 was replaced with a 32-bp target sequence from the gene ZMO0875

This study