|
| Microtube A | Microtube B | Microtube C |
| PCR Water | 8.5 μl | 8.5 μl | 8.5 μl |
| 10X Fast Digest Buffer | 1.0 μl | 1.0 μl | 1.0 μl |
| DNA | 4.0 μl | 4.0 μl | 4.0 μl |
| HinfI | 1.0 μl | 1.0 μl |
|
| ScrFI | 1.0 μl |
|
|
| MvaI |
| 1.0 μl |
|
| Bsh1236I |
|
| 2.0 μl |