Temp.

Time

Number of cycle

cDNA Synthesis

50˚C

15 min

1 Cycle

Thermo-Start Activation

95˚C

15 min

1 Cycle

Denaturation

95˚C

15 sec

40 Cycles

Annealing

50˚C - 60˚C

30 sec

Extension

72˚C

30 sec