Robustness Details

Factor

Level

Retention time

Asymmetry

%Assay

Flow rate

0.4

−1

5.67

1.15

99.86

0.6

0

5.14

1.15

99.64

0.8

+1

5.071

1.14

99.58

Mean ± SD (n = 3)

5.293 ± 0.327

1.146 ± 0.005

99.693 ± 0.147

Column Temperature

30

−1

5.19

1.18

99.49

35

0

5.14

1.15

99.82

40

+1

5.10

1.05

99.71

Mean ± SD (n = 3)

5.143 ± 0.0450

1.126 ± 0.068

99.673 ± 0.168

Buffer _PH

2.5

−1

5.03

1.2

98.9

2.7

0

5.14

1.15

99.68

2.9

+1

5.21

1.18

99.73

Mean ± SD (n = 3)

5.126 ± 0.090

1.176 ± 0.025

99.436 ± 0.465

% of Acetonitrile in the mobile phase

9

−1

5.21

1.13

99.85

10

0

5.14

1.15

99.68

11

+1

5.02

1.06

99.42

Mean ± SD (n = 3)

5.123 ± 0.096

1.113 ± 0.047

99.65 ± 0.216