Storage condition | Nominal concentration (ng/mL) | Measured concentration (ng/mL) | Recovery (%) |
Short term stability (6 h in plasma) | 0.30 | 109210* | 86.96 |
5.50 | - | - | |
8.80 | 3783601* | 98.33 | |
Autosampler stability after 65 h | 0.30 | 0.28 | 103.70 |
5.50 | - | - | |
8.80 | 9.41 | 97.82 | |
Freeze-thaw cycles (N = 5) | 0.30 | 0.28 | 93.33 |
5.50 | 6.09 | 110.73 | |
8.80 | 9.66 | 109.77 | |
Long term stability (198 day, −80˚C) | 0.30 | 0.27 | 91.11 |
5.50 | 5.62 | 102.12 | |
8.80 | 9.04 | 102.72 |