Parameters

METHOD-A

METHOD-B

Maximum wave length λ_max

414

635

Beer’s law Limits (µg/ml)

5.0 - 30.0

2.0 - 10.0

Optimum photometric Range µg/ml

12.7 - 26.02

3.6 - 7.5

Sandell’s sensitivity

0.0826

0.0111

Molar absorptivity lt/mole/cm

5.86 × 10⁴

8.35 × 10⁴

Slope (b)

0.114

0.348

Intercept (a)

0.0047

0.0055

Standard deviation on slope (S_b)

0.150

0.241

Standard deviation on Intercept (S_a)

0.0306

0.0302

Correlation coefficient (r)

0.9995

0.9996

Limit of detection (LOD) µg/ml

0.0561

0.0826

Limit of quantification (LOQ) µg/ml

0.1709

0.2504