Depth	Sample	Number of sequence reads	Observed number of OTUs^*	Estimated number of OTUs^* Chao 1	Diversity estimators		OTUs consisting of single sequences^*	OTUs consisting of double sequences^*	Observed number of OTUs, total	Estimated number of OTUs, total Chao 1	Diversity obtained from all sequences, %
Depth	Sample	Number of sequence reads	Observed number of OTUs^*	Estimated number of OTUs^* Chao 1	Shannon^*	Simpson^*	OTUs consisting of single sequences^*	OTUs consisting of double sequences^*	Observed number of OTUs, total	Estimated number of OTUs, total Chao 1	Diversity obtained from all sequences, %
0 m	a	3799	485	980	8.28	0.99	289	83	877	1161	75.6
0 m	b	10,822	488	1143	8.20	0.99	314	74	1275	1369	93.2
0.5 - 1 m	a	3621	351	924	6.95	0.98	235	47	686	1103	62.2
0.5 - 1 m	b	12,545	299	628	6.52	0.96	180	48	1149	1677	68.5
1.5 - 2 m	a	45,647	190	541	5.17	0.92	122	20	1830	2346	78.0
1.5 - 2 m	b	11,861	157	420	4.84	0.91	95	16	629	1109	56.7
2.5 - 3 m	a	10,177	185	465	5.66	0.96	109	20	671	1084	61.9
2.5 - 3 m	b	21,238	215	514	5.98	0.96	130	27	1089	1460	74.6
3.5 - 3.7 m	a	30							19	32	59.0
3.5 - 3.7 m	b	6442	183	523	5.53	0.95	108	16	469	795	59.0
3.7 - 4 m	a	1147	172	327	5.04	0.90	92	26	172	327	52.6
3.7 - 4 m	b	7768	177	372	4.76	0.86	103	26	5080	780	65.1
5.5 - 6 m	a	7698	281	568	6.50	0.97	168	48	814	1158	70.3
5.5 - 6 m	b	13,426	272	739	6.39	0.97	176	32	1073	1391	77.1
6.5 - 7 m	a	4359	355	682	7.26	0.98	202	61	655	801	81.7
6.5 - 7 m	b	7455	340	633	7.04	0.98	194	63	764	851	89.8