| Genes and primer names | Sequences | Annealing temperature (˚C) | Product size (bp) | Primer length (bp) |
| EGFR |
|
|
|
|
| EGFR-ex18-pF | 5'-CCGGAGTTTTCAATCCAGTT-3' | 64 | 278 | 20 |
| EGFR-ex18-pR | 5'-GTCAATGGCCCCTTTCATAA-3' | 20 | ||
| EGFR-ex19-pF | 5'-TGGATGAAATGATCCACACG-3' | 64 | 250 | 20 |
| EGFR-ex19-pR | 5'-AGTGCTGGGTAGATGCCAGT-3' | 20 | ||
| EGFR-ex20-pF | 5'-TCCGACTCCTCCTTTATCCA-3' | 64 | 337 | 20 |
| EGFR-ex20-pR | 5'-GATGGGACAGGCACTGATTT-3' | 20 | ||
| EGFR-ex21-pF | 5'-AAGTTCAAGCCCAGGTCTCA-3' | 58 | 346 | 20 |
| EGFR-ex21-pR | 5'-TCATTCACTGTCCCAGCAAG-3' | 20 | ||
| KRAS |
|
|
|
|
| Kras-ex1-pF | 5'-AAAGGTACTGGTGGAGTATTTG-3' | 58 | 265 | 22 |
| Kras-ex1-pR | 5'-TCTGTATCAAAGAATGGTCCTG-3' | 22 | ||
| Kras-ex2-pF | 5'-TCTTTGGAGCAGGAACAATG-3' | 58 | 400 | 20 |
| Kras-ex2-pR | 5'-TGCATGGCATTAGCAAAGAC-3' | 20 | ||
| RASSF1A |
|
|
|
|
| RASSF1A-M-pF | 5'-CGAGAGCGCGTTTAGTTTCGTT-3' | 58 | 169 | 22 |
| RASSF1A-M-pR | 5'-CGATTAAACCCGTACTTCGCTAA-3' | 23 | ||
| RASSF1A-U-pF | 5'-GGGGGTTTTGTGAGAGTGTGTTT-3' | 58 | 169 | 23 |
| RASSF1A-U-pR | 5'-CCCAATTAAACCCATACTTCACTAA-3' | 25 | ||
| CDKN2A |
|
|
|
|
| CDKN2A-M-pF | 5'-GGGTTGTTTTCGGTTGGTGTTTTC-3' | 68 | 150 | 24 |
| CDKN2A-M-pR | 5'-TCTAATAACCAACCAACCCCTCC-3' | 23 | ||
| CDKN2A-U-pF | 5'-GTGAGGGTTGTTTTTGGTTGGTGTTTTT-3' | 68 | 151 | 28 |
| CDKN2A-U-pR | 5'-TCTAATAACCAACCAACCCCTCC-3' | 23 | ||
| APC |
|
|
|
|
| APC-5-Ex-pF | 5'-TGGGYGGGGTTTTGTGTTTTATT-3' | 56 | 136 | 23 |
| APC-5-Ex-pR | 5'-TACRCCCACACCCAACCAATC-3' | 21 | ||
| APC-5-M-pF | 5'-TATTGCGGAGTGCGGGTC-3' | 61 | 98 | 18 |
| APC-5-M-pR | 5'-TCGACGAACTCCCGACGA-3' | 18 | ||
| APC-5-U-pF | 5'-GTGTTTTATTGTGGAGTGTGGGTT-3' | 58 | 108 | 24 |
| APC-5-U-pR | 5'-CCAATCAACAAACTCCCAACAA-3' | 22 | ||
| CDH1 |
|
|
|
|
| CDH1-M-pF | 5'-GGTGAATTTTTAGTTAATTAGCGGTAC-3' | 55 | 204 | 27 |
| CDH1-M-pR | 5'-CATAACTAACCGAAAACGCCG-3' | 21 | ||
| CDH1-U-pF | 5'-GGTAGGTGAATTTTAGTTAATTAGTGGTA-3' | 55 | 211 | 29 |
| CDH1-U-pR | 5'-ACCCATAACTAACCAAAAACACCA-3' | 24 | ||
| GATA-4 |
|
|
|
|
| GATA-4-M-pF | 5'-GTATAGTTTCGTAGTTTGCGTTTAGC-3' | 60 | 136 | 26 |
| GATA-4-M-pR | 5'-AACTCGCGACTCGAATCCCCG-3' | 21 | ||
| GATA-4-U-pF | 5'-TTTGTATAGTTTTGTAGTTTGTGTTTAGT-3' | 60 | 142 | 29 |
| GATA-4-U-pR | 5'-CCCAACTCACAACTCAAATCCCCA-3' | 24 | ||
| GATA-5 |
|
|
|
|
| GATA-5-M-pF | 5'-AGTTCGTTTTTAGGTTAGTTTTCGGC-3' | 60 | 140 | 26 |
| GATA-5-M-pR | 5'-CCAATACAACTAAACGAACGAACCG-3' | 25 | ||
| GATA-5-U-pF | 5'-TGGAGTTTGTTTTTAGGTTAGTTTTTGGT-3' | 60 | 147 | 29 |
| GATA-5-U-pR | 5'-CAAACCAATACAACTAAACAAACAAACCA-3' | 29 |