| Design points | Factors and levels | ANRF-1 | CHLRN-1 | ||||||
| X1 | X2 | X3 | Biomass | Lipid | % | Biomass | Lipid | % | |
| 1 | (−) | (−) | (−) | 18.2 | 5.0 | 27.6 | 45.3 | 16.5 | 36.5 |
| 2 | (+) | (−) | (−) | 25.6 | 4.9 | 18.5 | 73.6 | 13.3 | 18.8 |
| 3 | (−) | (+) | (−) | 21.4 | 6.0 | 28.3 | 45.0 | 13.2 | 29.4 |
| 4 | (+) | (+) | (−) | 30.4 | 5.0 | 16.2 | 85.0 | 15.1 | 17.7 |
| 5 | (−) | (−) | (+) | 15.5 | 4.0 | 26.2 | 21.6 | 11.0 | 49.8 |
| 6 | (+) | (−) | (+) | 18.5 | 6.0 | 31.6 | 70.0 | 11.5 | 16.4 |
| 7 | (−) | (+) | (+) | 14.0 | 3.0 | 21.2 | 42.3 | 11.6 | 27.4 |
| 8 | (+) | (+) | (+) | 18.2 | 6.0 | 33.3 | 80.6 | 12.0 | 14.8 |
| 9 | (0) | (0) | (0) | 24.1 | 7.8 | 32.6 | 56.0 | 14.4 | 25.8 |
| 10 | (0) | (0) | (0) | 21.0 | 6.4 | 30.9 | 60.3 | 10.0 | 16.6 |
| 11 | (0) | (0) | (0) | 24.0 | 7.3 | 30.8 | 65.0 | 16.3 | 25.2 |
| Level 0 (9 to 11) |
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| Average | 23.0 | 7.2 | 31.4 | 60.4 | 13.6 | 22.5 | |||
| DP | 1.8 | 0.7 | 1.0 | 4.5 | 3.2 | 5.15 | |||
| Control |
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| ASM−1 | 36.8 | 6.4 | 18.0 | 88.0 | 9.6 | 10.9 | |||