Steps | Elute (ml) | Activity (iU/L) | Total activity (IU) | Protein concentration (mg/L) | Total protein (mg) | Specific activity (iU/mg) | Purification (folds | Yield% |
Crude | 7.5 | 73.02 | 0.547 | 51.4 | 0.386 | 1.419 | - | 100 |
Ammonium sulphate | 5.5 | 60.3 | 0.332 | 30.2 | 0.166 | 1.996 | 1.406 | 60.62 |
Dialysis | 5 | 41.21 | 0.206 | 8.7 | 0.0435 | 4.737 | 3.338 | 37.66 |
Concentrated | 4.5 | 66.54 | 0.299 | 14 | 0.063 | 4.752 | 3.349 | 54.73 |
Gel filtration | 5 | 51.82 | 0.259 | 10 | 0.05 | 5.18 | 3.65 | 47.34 |
Ion exchange DEAE-Cellulose A50 Isoenzyme-I | 3 | 38.1 | 0.114 | 4.15 | 0.0124 | 9.209 | 6.490 | 20.87 |
Isoenzyme-II | 3 | 16.363 | 0.049 | 2.2 | 0.0066 | 7.424 | 5.232 | 8.95 |