Investigated Parameters

Evaluated Levels

Solvent composition

Alamine^® 336

(% w/v)

0; 10; 20; 30; 40; 50

or (% w/w)

0; 12.4; 25.0; 37.5; 50.0; 62.4

Exxal^™ 13 tridecyl alcohol

(% w/v)

2; 8; 10; 15; 20

or (% w/w)

2.6; 10.3; 12.6; 19.0; 25.0

Time (min)

1; 3; 5; 7;10; 20

Fixed Parameters

Levels

Temperature (˚C)

28 ± 2

Volumetric ratio (A/O)

1/1

Stirring speed (rpm)

400