Groups

Grades

Reactivity

Conditions of all cultures

Vehicle control

0

None

Discrete intracytoplasmatic granules, no cell lysis, no reduction of cell growth.

HDPE control

1

Slight

Not more than 20% of the cells are round, loosely attached and without intracytoplasmatic granules, or show changes in morphology; occasional lysed cells are present; only slight growth inhibition observable.

Silver based dressing control extracts

1

Slight

Not more than 20% of the cells are round, loosely attached and without intracytoplasmatic granules, or show changes in morphology; occasional lysed cells are present; only slight growth inhibition observable.

AgNO₃ solution (3.5 µg/mL)

3

Moderate

Not more than 70% of the cell layers contain rounded cells or are lysed; cell layers not completly destroyed, but more than 50% growth inhibition observable.

Silver based dressing extracts (3.8 µg/mL)

3

Moderate

Not more than 70% of the cell layers contain rounded cells or are lysed; cell layers not completly destroyed, but more than 50% growth inhibition observable.

SPU-ZDEC extracts (100%)

4

Severe

Nearly complete or complete destruction of the cell layers.