Gene | Description | Primer | Tm |
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase (reference gene) | Forward: 5’ TCGGTGTGAACGGATTTGG 3’ Reverse: 5’ TCTCCACTTTGCCACTGCA 3’ | 55.4C 57.1C |
BGLAP | Osteocalcin | Forward: 5’ CTGACAAAGCCTTCATGTCCAA 3’ Reverse: 5’ GCCGGAGTCTGTTCACTACCTT 3’ | 55.6C 58.7C |
Runx | Transcription Factor RunX2 | Forward: 5’ CTTCAAGGTTGTAGCCCTCGGA 3’ Reverse: 5’ ATGACGGTAACCACAGTCCCA 3’ | 58.8C 58.2C |
AP | Alkaline Phosphatase | Forward: 5’ AAGGATATCGACGTGATCATGG 3’ Reverse: 5’ GGCCTTCTCATCCAGTTCGTA 3’ | 54.3C 56.5C |
SMAD4 | SMAD family member 4 | Forward: 5’ CACAAGTCAGCCGGCCAGTATT 3’ Reverse: 5’ TTCCAGTCCAGGTGGTAGT 3’ | 59.8C 60.1C |