Gene

Description

Primer

Tm

GAPDH

Glyceraldehyde 3-phosphate dehydrogenase (reference gene)

Forward: 5’ TCGGTGTGAACGGATTTGG 3’

Reverse: 5’ TCTCCACTTTGCCACTGCA 3’

55.4C

57.1C

BGLAP

Osteocalcin

Forward: 5’ CTGACAAAGCCTTCATGTCCAA 3’

Reverse: 5’ GCCGGAGTCTGTTCACTACCTT 3’

55.6C

58.7C

Runx

Transcription Factor RunX2

Forward: 5’ CTTCAAGGTTGTAGCCCTCGGA 3’

Reverse: 5’ ATGACGGTAACCACAGTCCCA 3’

58.8C

58.2C

AP

Alkaline Phosphatase

Forward: 5’ AAGGATATCGACGTGATCATGG 3’

Reverse: 5’ GGCCTTCTCATCCAGTTCGTA 3’

54.3C

56.5C

SMAD4

SMAD family member 4

Forward: 5’ CACAAGTCAGCCGGCCAGTATT 3’

Reverse: 5’ TTCCAGTCCAGGTGGTAGT 3’

59.8C

60.1C