Analyzes | Analysis method and type of device | ||
Methods | Material | References | |
Dry matter (%) | Drying at 105˚C, 2 hours | Oven (Memmert, ULE 500, Germany) | [16] |
Ash (mg/100g) | Incineration at 550˚C, 4 hours | Moufle oven (Carbolite, OAF 11/1, England) | [17] |
pH at 25˚C | pH-metry | pH meter (Hanna Instruments, HI 223, USA) | [18] |
Turbidity | Turbidimetry | Turbidimeter |
|
Volumic mass | Weighing | Pycnometer |
|
Titratable acidity (mEq/100g) | Titrimetry | Potentiometer titrator (SI Analytics, TitroLine easy, Germany) | [19] |
BRIX (%) | Refractometry | Refractometer |
|
Dietary fiber (%) | Gravimetry | Common laboratory equipment | [20] |
Total sugars (g/100g) | Luff-Schoorl after hydrolysis | Common laboratory equipment | [21] |
Total carotenoids (mg carotene/100g) | Spectrophotometry | UV/Visible Spectrophotometer (Analytik Jena, Specord 200 plus, Germany) | [22] |
Total polyphenols (mg gallic acid/100g) | Follin-Ciocalteu | UV/Visible Spectrophotometer (Analytik Jena, Specord 200 plus, Germany) | [23] |
Total Tannins (mg tannic acid/100g) | Follin -Denis | UV/Visible Spectrophotometer (Analytik Jena, Specord 200 plus, Germany) | [24] |
Flavonoids (mg catechin acid/100g) | Colorimetric | UV/Visible Spectrophotometer (Analytik Jena, Specord 200 plus, Germany) | [25] |
Antioxidant activity (% inhibition) at 0.1 mg/L sample | DPPH | UV/Visible Spectrophotometer (Analytik Jena, Specord 200 plus, Germany) | [25] |