| Instrument | Shimadzu LC system | |||
| Sampler temp. | 10˚C | |||
| Injection volume | 20 μL | |||
| Rinse solution | Water | |||
| Mobile phase | Mobile Phase A | |||
| Mobile Phase B | ||||
| Time (min) | A (%) | B (%) | ||
| 0 | 100 | 0 | ||
| Flow rate | 0.3 mL/min | |||
| Column | Aminex HPX-87P 300*7.8 mm | |||
| Column oven temp. | 85˚C | |||
| Detector | Refractive index (RI), 55˚C | |||