| Instrument | Shimadzu LC system | |||
| Sampler temp. | 10˚C | |||
| Injection volume | 20 μL | |||
| Rinse solution | 50% MeOH | |||
| Mobile phase | Mobile Phase A | IPA | ||
|
| Mobile Phase B | Hexane (1% acetic acid) | ||
|
| Gradient | Time (min) | A (%) | B (%) |
|
| 0 | 5 | 95 | |
|
| 10 | 5 | 95 | |
|
| 11 | 100 | 0 | |
|
| 15 | 100 | 0 | |
|
| 16 | 5 | 95 | |
|
| 30 | 5 | 95 | |
| Flow rate | 0.5 mL/min | |||
| Column | Shimadzu Shim-pack GIS CN 150 × 4.6 mm 5 μm | |||
| Column oven temp. | 30˚C | |||
| Detector | Photodiode array (PDA), 320 nm | |||