Wave number (cm−1)

Functional Group Assignments

3500 - 3700

O-H stretching vibration of hydroxyl groups (mainly lipids and proteins)

3380

O-H stretching, Amine (N-H stretching vibration) mainly carbohydrates proteins

2957

CH3 asym stretching (mainly lipids)

2922

CH2 asym stretching (mainly lipids)

2873

CH3 sym stretching (mainly proteins)

2852

CH2 sym stretching (mainly lipids)

1710, 1733

C=O carbonyl stretching of saturated aliphatic esters

1630

Amide I (protein C=O stretch)

1555

Amide II (C-N, N-H stretching) mainly proteins

1415

O-H bending, polysaccarides

1377

Symmetric bending of aliphatic CH3, triterpene compounds (CH2=CH-CH3)

1250

Pectic substances

1235

Amide III (C-N, N-H stretching) mainly proteins

1145

Cellulose (b-glucan), triterpene compounds (C-O)

1101

Antisym in-phase, pectic substances

1073

Rhamnogalactorunan, b-galactan

1064

C-O stretching , cell wall polysaccharides (glucomannan)

1035

OH and C-OH stretching in sugars, cell wall polysaccharides (arabinan)