Trial | Experimental design | Experimental set up | ||||
X1 | X2 | X3 | Organic modifier (%) | Flow rate (mL/min) | Temperature (˚C) | |
1 | −1 | −1 | 0 | 80 | 0.3 | 30 |
2 | 1 | 0 | 1 | 90 | 0.5 | 35 |
3 | 0 | 0 | 0 | 85 | 0.5 | 30 |
4 | 1 | −1 | 1 | 90 | 0.3 | 35 |
5 | 0 | −1 | 1 | 85 | 0.3 | 35 |
6 | −1 | 1 | 1 | 80 | 0.7 | 35 |
7 | −1 | 1 | −1 | 80 | 0.7 | 25 |
8 | 1 | −1 | 0 | 90 | 0.3 | 30 |
9 | 0 | 0 | 0 | 85 | 0.5 | 30 |
10 | 0 | 0 | 0 | 85 | 0.5 | 30 |
11 | −1 | −1 | −1 | 80 | 0.3 | 25 |
12 | 1 | 1 | −1 | 90 | 0.7 | 25 |
13 | −1 | 1 | 0 | 80 | 0.7 | 30 |
14 | −1 | 0 | 1 | 80 | 0.5 | 35 |
15 | 1 | 0 | −1 | 90 | 0.5 | 25 |
16 | 0 | 0 | −1 | 85 | 0.5 | 25 |
17 | 0 | 0 | 1 | 85 | 0.5 | 35 |
18 | 0 | −1 | 1 | 85 | 0.3 | 35 |
19 | 0 | −1 | −1 | 85 | 0.3 | 25 |
20 | 0 | 1 | 1 | 85 | 0.7 | 35 |
21 | −1 | 0 | 0 | 80 | 0.5 | 30 |
22 | 1 | 0 | 0 | 90 | 0.5 | 30 |
23 | 1 | 1 | 1 | 90 | 0.7 | 35 |
24 | 0 | −1 | 0 | 85 | 0.3 | 30 |
25 | 1 | −1 | −1 | 90 | 0.3 | 25 |
26 | 0 | 1 | 0 | 80 | 0.7 | 30 |
27 | −1 | −1 | 1 | 85 | 0.3 | 35 |
28 | 1 | 1 | 0 | 90 | 0.7 | 30 |
29 | −1 | 0 | −1 | 80 | 0.5 | 25 |