Trial

Experimental design

Experimental set up

X1

X2

X3

Organic modifier (%)

Flow rate (mL/min)

Temperature (˚C)

1

−1

−1

0

80

0.3

30

2

1

0

1

90

0.5

35

3

0

0

0

85

0.5

30

4

1

−1

1

90

0.3

35

5

0

−1

1

85

0.3

35

6

−1

1

1

80

0.7

35

7

−1

1

−1

80

0.7

25

8

1

−1

0

90

0.3

30

9

0

0

0

85

0.5

30

10

0

0

0

85

0.5

30

11

−1

−1

−1

80

0.3

25

12

1

1

−1

90

0.7

25

13

−1

1

0

80

0.7

30

14

−1

0

1

80

0.5

35

15

1

0

−1

90

0.5

25

16

0

0

−1

85

0.5

25

17

0

0

1

85

0.5

35

18

0

−1

1

85

0.3

35

19

0

−1

−1

85

0.3

25

20

0

1

1

85

0.7

35

21

−1

0

0

80

0.5

30

22

1

0

0

90

0.5

30

23

1

1

1

90

0.7

35

24

0

−1

0

85

0.3

30

25

1

−1

−1

90

0.3

25

26

0

1

0

80

0.7

30

27

−1

−1

1

85

0.3

35

28

1

1

0

90

0.7

30

29

−1

0

−1

80

0.5

25