Gene (accession no.)

Nucleotide sequence (5' - 3')

Product size (bp)

Annealing temperature (˚C)

AgRP

215

58

Forward

AGA CAT ATT CCC TGC GCT GA

Reverse

GCA GCT TTG AAT CCT GCT CT

Ghrelin = GHRL

194

60

Forward

GCA TCC TCA AAG ACC AGG AG

Reverse

CTT GAC ACT GGG GTT CCA CT

GAPDH

294

58

Forward

AAG GGT GGA GCC AAA AGG

Reverse

GGA TGC AGG GAT GAT GTT CT