Pre-analytical error | · Incorrect sampling method and sampling location · Inappropriate material for sampling · Inadequate sample volume · Inadequate or improper use of PPE · Lack of human resources |
Analytical error | · Low quantity and quality of extracted RNA · RNA contamination during extraction · Pipetting errors · Use of inappropriate sample volume for cDNA synthesis and · Improper storage of PCR reagents |
Post-analytical error | · Misinterpretation · Incorrect determination of exact baseline and threshold · Amplicon contamination |