| Volume of ammonia (mL) | pH of growth solution | Quantity of NH3 (mmol) |
| 2 | 10.38 | 26.6 - 29.6 |
| 2.5 | 10.59 | 33.3 - 37.0 |
| 3 | 10.73 | 40.0 - 44.4 |
| 3.5 | 10.84 | 46.7 - 51.8 |
| 4 | 10.88 | 53.3 - 59.2 |