Gene	Forwardprimer			Reverse primer			Annealing temperature
Gene	Location	ID	Sequence	Location	ID	Sequence	Annealing temperature
EuCesA1	Promoter	EgCesA1_-276.22F	GCTTCAACACA ATGACACCAAC	Exon 2	EgCesA1_212.23R	AATTCATTTCTC TCAGCATCAGC	59
	Exon 1	EgCesA1_108.22F	CAAGGCTTGTG TCGAATATGAG	Exon 4	EgCesA1_421.22R	GAACTTGAGCC TCTTTCTCAGC	62
	Exon 3	EgCesA1_275.23F	GACACATCAGC AGTGTTTCTACG	Exon 5	EgCesA1_693.23R	AGTAATCCTAT TCACGGGAGACC	59
	Exon 5	EgCesA1_629.20F	TTTGGTTCGCAT ACTCATGG	Exon 6	EgCesA1_918.23R	CGTTTCAACAAG AGACTCGAATG	59
	Exon 6	EgCesA1_796.22F	CCTTTGATCACT GCCAATACAG	Exon 9	EgCesA1_1379.22R	CAGTCAAGGTT GAGGATGTACG	59
	Exon 8	EgCesA1_1245.20F	ACTTCCTCGGC TGGTGTATG	Exon 11	EgCesA1_1892.23R	AACAGCGTCGA CTCAATAAACAC	62
	Exon 10	EgCesA1_1636.22F	TCTATGCCGAA TTTACCAAAGC	Exon 12	EgCesA1_2310.23R	AATTGTGCAATA AGCGACAAGAG	59
	Exon 12	EgCesA1_2167.25F	TCCGTGGAAATA TTCTTGAGTAGAC	Exon 13	EgCesA1_2706.21R	ATAACCTTTGT TCAGCGCATC	59
	Exon 13	EgCesA1_2518.22F	GGATTCCTGAAG ATGTTAGCTG	Exon 13 (3'UTR)	EgCesA1_3214.20R	ATTCCATGCAT CGCACATTC	59
EuCesA2	Promoter	EgCesA2_-152.22F	TGACGTCTCTTC AACTCAAACC	Exon 2	EgCesA2_144.18R	GTCCTTCAGG CCGATCTC	62
	Exon 2	EgCesA2_124.18F	GACGAGATC GGCCTGAAG	Exon 3	EgCesA2_339.21R	AAACTCGTCC TCGAAATCCTC	62
	Exon 3	EgCesA2_295.19F	GACGAGGAC GACCACTTCG	Exon 5	EgCesA2_597.22R	GTAGTCATCTT CCTCTCCATCG	54
	Exon 5	EgCesA2_495.20F	GGAGTGGAA GGAGAGGATCG	Exon 6	EgCesA2_843.23R	AGTTTCTCTGTC TATCGGGTTCC	54
	Exon 6	EgCesA2_735.22F	AACTGATGCA TTCCCTCTATGG	Exon 6	EgCesA2_1184.21R	CTGGGCTCCAC CTTATCTTTC	60
	Exon 6	EgCesA2_1108.20F	TATAGCATCG AGCCGAGGAC	Exon 9	EgCesA2_1615.22R	CATAGCAAAG CTTCTTTCCAAG	54
	Exon 8	EgCesA2_1385.21F	AAGGTAAGG AGTTGCCTCGAC	Exon 10	EgCesA2_2008.23R	AACCCTCAAGT CCTTCTTCAATG	57
	Exon 10	EgCesA2_1878.23F	GAAGGATGAT ACGAGTTTGCTTG	Exon 11	EgCesA2_2488.23R	TGGAAGTGAAA GGGTAGACAATG	60
	Exon 11	EgCesA2_2399.22F	ATTGTCCTTTG TGGTATGCTTG	Exon 12	EgCesA2_2917.23R	ACGATCCGTAT CCATTGTTTATG	60