Compounds

Rf in CCM (×100)

HPLC

LC-MS

TLC-MS

Acetonitrile—water—TFA

t_{R (min)}

m/z

m/z

1

24

15.5

[M]⁺ 493; [M-162] 331

[M]⁺ 493; [M-162] 331

2

28

14.5

[M]⁺ 463; [M-162] 301

3

33

13

[M]⁺ 479; [M-162] 317

[M]⁺ 479; [M-162] 317

4

38

12

[M]⁺ 449; [M-162] 287

[M]⁺ 449; [M-162] 287

5

43

10.5

[M]⁺ 465; [M-162] 303

[M]⁺ 465; [M-162] 303