Products | Primers | Genes (bp) | Sequences (5’-3’) | PCR conditions | References |
16S rRNA | 27F1 1494 Rc | rrn (1467) | AGAGTTTGATCCTGGCTCAG TACGGCTACCTTGTTACGAC | 95˚C/3min; 30 cycles (94˚C/10s, 50˚C/20s, 72˚C/1min); 72˚C/7min | Neilan et al., 1997 |
16S rRNA | CYA359F CYA781Ra CYA781Rb | rrn (422) | GGGGAATTTTCCGCAATGGG GACTACTGGGGTATCCTAATCCCATT GACTACAGGGGTATCTAATCCCTTT | 94˚C/5min; 35 cycles (94˚C/1min, 63˚C/1min, 72˚C/1min); 72˚C/7min | Nüben et al., 1997 |
Anatoxin | ALM_F ALM_R | anaC (421) | CTGGGGGCACAATTATTTTACTCTCACCTG ACGCAAAGCTCACCCACCTCACCTCTAACT | 94˚C/4min; 30 cycles (94˚C/20s, 45˚C/20s, 72˚C/1min); 72˚C/7min | Méjean et al., 2009 |
Cylindrospermopsin | CYLAT-F CYLAT-R | cyrA (1100) | ATTGTAAATAGCTGGAATGAGTGG TTAGGGAAGTAATCTTCACAG | 94˚C/3min; 30 cycles (94˚C/10s, 50˚C/20s, 72˚C/1min); 72˚C/7min | Kellmann et al., 2006 |
Cylindrospermopsin | cynsulf-F cylnam-R | cyrJ (780) | CTTCTCTCCTTTCCCTAT GAGTGAAAATGCGTAGAACTTG | 94˚C/3min; 30 cycles (94˚C/10s, 50˚C/20s, and 72˚C/1min); 72˚C/7min | Mihali et al., 2008 |
Microcystin | mcyE-F2 mcyE-R4 | mcyE (810) | GAAATTTGTGTAGAAGGTGC AATTCTAAAGCCCAAAGACG | 95˚C/3min; 30 cycles (94˚C/30s, 56˚C/30s, and 72˚C/1min); 72˚C/10min | Rantala et al., 2004 |
Saxitoxin | OCT-F OCT-R | sxtI (923) | TGCCGTTTTGTGCTTAGATG GGACGGAAGGACTCACGATA | 94˚C/5min; 35 cycles (94˚C/30s, 61˚C/1min, and 72˚C/1min); 72˚C/7min | Fiore et al., 2010 |
Aeruginosin | aerA_F aerB_R | aerA-aerB intergenic region (991) | GATAGCACCCAGAACGGAAGC CGTTAAACGGATGGTTAGAGC | 95˚C/2min; 40 cycles (95˚C/45s, 50˚C/45s, and 72˚C/1min); 72˚C/7min | Cadel-Six et al., 2008 |
Cyanopeptolin | mcnC_F mcnE_R | mcnC-mcnE intergenic region (585) | TAAGGATAATTTCTTTGAATTGGGAG GGGAATAATCTCTAAATCAACAGC | 95˚C/2min; 40 cycles (95˚C/45s, 50˚C/45s, and 72˚C/1min); 72˚C/7min | Cadel-Six et al., 2008 |