Products

Primers

Genes (bp)

Sequences (5’-3’)

PCR conditions

References

16S rRNA

27F1

1494 Rc

rrn (1467)

AGAGTTTGATCCTGGCTCAG

TACGGCTACCTTGTTACGAC

95˚C/3min; 30 cycles (94˚C/10s, 50˚C/20s, 72˚C/1min); 72˚C/7min

Neilan et al., 1997

16S rRNA

CYA359F

CYA781Ra CYA781Rb

rrn (422)

GGGGAATTTTCCGCAATGGG

GACTACTGGGGTATCCTAATCCCATT

GACTACAGGGGTATCTAATCCCTTT

94˚C/5min; 35 cycles (94˚C/1min, 63˚C/1min, 72˚C/1min); 72˚C/7min

Nüben et al., 1997

Anatoxin

ALM_F

ALM_R

anaC (421)

CTGGGGGCACAATTATTTTACTCTCACCTG

ACGCAAAGCTCACCCACCTCACCTCTAACT

94˚C/4min; 30 cycles (94˚C/20s, 45˚C/20s, 72˚C/1min); 72˚C/7min

Méjean et al., 2009

Cylindrospermopsin

CYLAT-F

CYLAT-R

cyrA (1100)

ATTGTAAATAGCTGGAATGAGTGG

TTAGGGAAGTAATCTTCACAG

94˚C/3min; 30 cycles (94˚C/10s, 50˚C/20s, 72˚C/1min); 72˚C/7min

Kellmann et al., 2006

Cylindrospermopsin

cynsulf-F

cylnam-R

cyrJ (780)

CTTCTCTCCTTTCCCTAT

GAGTGAAAATGCGTAGAACTTG

94˚C/3min; 30 cycles (94˚C/10s, 50˚C/20s, and 72˚C/1min); 72˚C/7min

Mihali et al., 2008

Microcystin

mcyE-F2

mcyE-R4

mcyE (810)

GAAATTTGTGTAGAAGGTGC

AATTCTAAAGCCCAAAGACG

95˚C/3min; 30 cycles (94˚C/30s, 56˚C/30s, and 72˚C/1min); 72˚C/10min

Rantala et al., 2004

Saxitoxin

OCT-F

OCT-R

sxtI (923)

TGCCGTTTTGTGCTTAGATG

GGACGGAAGGACTCACGATA

94˚C/5min; 35 cycles (94˚C/30s, 61˚C/1min, and 72˚C/1min); 72˚C/7min

Fiore et al., 2010

Aeruginosin

aerA_F

aerB_R

aerA-aerB intergenic region (991)

GATAGCACCCAGAACGGAAGC

CGTTAAACGGATGGTTAGAGC

95˚C/2min; 40 cycles

(95˚C/45s, 50˚C/45s, and 72˚C/1min); 72˚C/7min

Cadel-Six et al., 2008

Cyanopeptolin

mcnC_F

mcnE_R

mcnC-mcnE intergenic region (585)

TAAGGATAATTTCTTTGAATTGGGAG

GGGAATAATCTCTAAATCAACAGC

95˚C/2min; 40 cycles

(95˚C/45s, 50˚C/45s, and 72˚C/1min); 72˚C/7min

Cadel-Six et al., 2008