Step | Temperature | Time | Number of Cycles |
Initial Denaturation | 95˚C | 2 minutes | 1 cycle |
Denaturation | 95˚C | 0.5 - 1 minute | 25 - 35 cycles |
Annealing | 42˚C - 65˚C* | 0.5 - 1 minute | 25 - 35 cycles |
Extension | 72˚C | 1 minutes/kb | 25 - 35 cycles |
Final Extension | 72˚C | 5 minutes | 1 cycle |
Soak | 4˚C | indefinite | 1 cycle |