| Primer name | Sequence (5'-3') | Length | Usage | Annealing temperature (˚C) |
| Oligo dT | CTGTGAATGCTGCGACTACGATTTTTTTTTTTTTTTTTTT | 40 mer | cDNA synthesis | |
| Chi3 F1 (F) | TGYTAYTTYACNAAYTGG | 19 mer | Conserved region PCR | 50.0 |
| Chi3 F2 (F) | GAYATHGAYTGGGARTAYCC | 19 mer | ||
| Chi3 R (R) | TTCCARTARTTCATNGCRTARTC | 23 mer | ||
| 3'RACE (R) | CTGTGAATGCGACTACGAT | 19 mer | 3' RACE PCR | 50.0 |
| Chi3 3' F1 (F) | AAGAGGAAGTCCTCTGGAGG | 20 mer | ||
| Chi3 3' F2 (F) | GCTGAGGGAACAGCAACTGG | 20 mer | ||
| Chi3 5' R1 (R) | GCAGCGGAGACCATTAATC | 19 mer | 5' RACE PCR | 50.0 |
| Chi3 5' R2 (R) | CCAGTTGCTGTTCCCTCA | 18 mer | ||
| Chi3 5' F1 (F) | AGGAACCATTGATGCTGGTT | 20 mer | ||
| Chi3 5' F2 (F) | TGGCAAAGTACCTGGACTT | 19 mer | ||
| Chi3 5' F3 (F) | CGTGATGACGTATGACTTTCATG | 23 mer | ||
| Chi3 5' P | GTGATGTCCTGTCACGCT | 18 mer | ||
| Chi3 full F (F) | GACATGACAAGAGACCATCCAA | 22 mer | Full length amplification | 55.0 |
| Chi3 full R (R) | ACAGAGCATTTCAAACAGAGGG | 22 mer | ||
| β-actin (F) | GATCATGTTCGAGACCTTCAACAC | 24 mer | Organ expression | 50.0 |
| β-actin (R) | TCCAATCCAGACAGAGTATTTAGC | 24 mer | ||
| SmChi-1 (F) | ACTAGCGTGATCAAGTTCC | 19 mer | ||
| SmChi-1 (R) | ACAGCTCCAATCGCGGA | 17 mer | ||
| SmChi-2 (F) | CTCAGTCATTTAATTCCTGA | 20 mer | ||
| SmChi-2 (R) | CCTGGCCAAGCTTGGGAAT | 19 mer | ||
| SmChi-3 (F) | CAGTCTTCTGTCAACCTAC | 19 mer | ||
| SmChi-3 (R) | GGTACTTTGCCATCTCTGC | 19 mer |