A. Units recorded from all animals

Dose:

Acute Day 1

Baseline Day 10

Rechallenge Day 10

N

↑

↓

≠

↑

↓

≠

↑

↓

≠

saline

45

2

(4%)

3

(7%)

40 (89%)

0

(0%)

2

(4%)

43

(96%)

1

(2%)

3

(7%)

41 (91%)

0.6 mg/kg MPD

147

32

(20%)

21

(14%)

94 (64%)

31

(21%)

31

(21%)

85

(58%)

52

(36%)

18

(12%)

77 (52%)

2.5 mg/kg MPD

165

69

(42%)

35

(21%)

61 (37%)

82

(50%)

23

(14%)

60

(86%)

93

(56%)

22

(13%)

50 (31%)

10.0 mg/kg MPD

102

61

(60%)

18

(18%)

23 (22%)

69

(68%)

16

(17%)

17

(15%)

63

(62%)

20

(20%)

19 (18%)

B. Units recorded from behaviorally sensitized animals

Dose:

N

Acute Day 1

Baseline Day 10

Rechallenge Day 10

↑

↓

≠

↑

↓

≠

↑

↓

≠

0.6 mg/kg MPD

62

20

(32%)

19

(31%)

23 (37%)

37

(60%)

14

(23%)

11

(17%)

14

(23%)

38

(61%)

10 (16%)

2.5 mg/kg MPD

103

16

(16%)

52

(50%)

35 (34%)

24

(23%)

53

(52%)

26

(25%)

22

(21%)

71

(69%)

10 (10%)

10.0 mg/kg MPD

74

15

(20%)

47

(64%)

12 (16%)

8 (11%)

61

(82%)

5

(7%)

10

(13%)

53

(72%)

11 (15%)

C. Units recorded from behaviorally tolerant animals

Acute Day 1

Baseline Day 10

Rechallenge Day 10

Dose:

N

↑

↓

≠

↑

↓

≠

↑

↓

≠

0.6 mg/kg MPD

85

8

(9%)

17

(20%)

60 (71%)

6

(7%)

13

(15%)

66

(78%)

19

(22%)

4

(5%)

62 (73%)

2.5 mg/kg MPD

62

25

(40%)

14

(23%)

23 (37%)

33

(53%)

6 (10%)

23

(37%)

39

(63%)

6 (10%)

17 (27%)

10.0 mg/kg MPD

28

15

(54%)

9 (32%)

4 (14%)

15

(54%)

7 (25%)

6

(21%)

15

(54%)

5 (18%)

8 (28%)