Fluorescence parameters derived from the extracted data | |||
dV/dto = 4(F300µs − F0)/(FM − F0) | Approximated initial slope (in ms−1) of the fluorescence transient V = f(t) | ||
Sm = (Area)/(FM − F0) | Normalized total complementary area above the OJIP transient (reflecting multiple turnover QA reduction events) | ||
Yields or flux ratios | |||
TRo/ABS = [1 − F0/FM] | Maximum quantum yield of primary photochemistry at t = 0 | ||
jEo= ETo/ABS = [1 − (FJ/FM)] | Quantum yield for electron transport at t = 0 | ||
yo = ETo/TRo = (1 − VJ) | Probability (at t = 0) that a trapped exciton moves an electron into the electron transport chain beyond QA- | ||
Specific fluxes or activities per reaction center (RC) | |||
10RC/ABS = Mo(1/VJ)(1/jPo) | Absorption per RC | ||
TRo/RC = Mo/VJ | Trapped energy flux per RC (at t = 0) | ||
ETo/RC = Mo(1/VJ)yo | Electron transport flux per RC (at t = 0) | ||
Phenomenological fluxes or activities per excited cross section (CS) | |||
ABS/CSo » F0 | Absorption flux per CS, approximated by F0 | ||
TRo/CS = jPo(ABS/CSo) | Trapped energy flux per CS (at t = 0) | ||
ETo/CS = jEo(ABS/CSo) | Electron transport flux per CS (at t = 0) | ||
De-excitation rate constants | |||
Kp | Photochemical de-excitation rate constant | ||
Kn | Non-photochemical de-excitation rate constant | ||
Sum K | The sum of photochemical and non-photochemical rate constants | ||
Performance index | |||
PI = RC/(ABSRC) × jPo/(1 − jPo) × yo/(1 − yo) | Performance index on absorption basis | ||