| Treatments | Absorbance at 450 nm first analysis | Absorbance at 450 nm second analysis | Relative quantification of methylated DNA (%) |
| 1 | 0.615 | 0.661 | 16.62 ab |
| 2 | 0.637 | 0.811 | 18.75 a |
| 3 | 0.370 | 0.479 | 10.99 b |
| 4 | 0.714 | 0.886 | 20.74 a |
| 5 | 0.670 | 0.900 | 20.29 a |
| 6 | 0.835 | 1.115 | 25.21 a |
| Methylated DNA control | 3.586 | 4.109 | - |
| CV% | - | - | 5.61 |