| Name | Ion Torrent Linker Primer | Golay Barcode | Spacer | 16S rRNA Primer |
| Forward Primer(s) |
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| A341F_BACT_A_21 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-GATCTGCGATCC-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_22 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-CAGCTCATCAGC-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_23 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-CAAACAACAGCT-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_24 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-GCAACACCATCC-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_25 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-GCGATATATCGC-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_26 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-CGAGCAATCCTA-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_27 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-AGTCGTGCACAT-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_28 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-GTATCTGCGCGT-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_29 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-CGAGGGAAAGTC-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_30 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-CAAATTCGGGAT-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_31 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-AGATTGACCAAC-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_32 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-AGTTACGAGCTA-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_33 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-GCATATGCACTG-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_34 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-CAACTCCCGTGA-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_35 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-TTGCGTTAGCAG-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_36 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-TACGAGCCCTAA-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_37 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-CACTACGCTAGA-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| A341F_BACT_A_38 | 5’-CCATCTCATCCCTGCGTGTCTCCGACTCAG-3’ | 5’-TGCAGTCCTCGA-3 | GT | 5’-CCTACGGGAGGCAGCAG-3’ |
| Reverse Primer |
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|
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| 518R_BACT_P1 | 5’-CCTCTCTATGGGCAGTCGGTGAT-3’ | Not applicable | CC | 5’-CGACAGCCATGCANCACCT |