| Standard PCR | Direct colony PCR | ||
Steps | U$ | Time | U$ | Time |
Bacterial isolation on plate | 0.31 | 24 - 72 h | 0.31 | 24 - 72 h |
Replication in broth | 0.41 | 24 - 48 h | - | - |
DNA extraction | 5.6 | 1 - 3 h | - | - |
PCR | 2.52 | 3 h | 2.52 | 3 h |
Electrophoresis | 0.27 | 1.5 h | 0.27 | 1.5 h |
PCR product purification | 2.54 | 1 h | - | - |
16S rRNA sequencing | 5.0 | 24 h | 5.0 | 24 h |
Nucleotide analysis | - | 1 h | - | 1 h |
Overall | 16.65 | 4 - 7 days | 8.1 | 3 - 5 days |