Standard PCR

Direct colony PCR

Steps

U$

Time

U$

Time

Bacterial isolation on plate

0.31

24 - 72 h

0.31

24 - 72 h

Replication in broth

0.41

24 - 48 h

-

-

DNA extraction

5.6

1 - 3 h

-

-

PCR

2.52

3 h

2.52

3 h

Electrophoresis

0.27

1.5 h

0.27

1.5 h

PCR product purification

2.54

1 h

-

-

16S rRNA sequencing

5.0

24 h

5.0

24 h

Nucleotide analysis

-

1 h

-

1 h

Overall

16.65

4 - 7 days

8.1

3 - 5 days