Conditions | Conventional spectrophotometric method | Microplate method |
NQS working conc. (M) | 0.05 | 0.05 |
NQS volume (μl) | 2000 | 50 |
NaOH working conc. (M) | 0.25 | 0.25 |
NaOH volume (μl) | 2000 | 50 |
Reaction time (min) | 10 | 10 |
Temperature (˚C) | 25 ± 2 | 25 ± 2 |
Solvent | Dist. water | Dist. water |
Total volume of final solution μl) | 10,000 | 200 |
Measuring instrument | UV-VIS Spectrophotometer | Microplate reader |
Wavelength of measurements (nm) | 472 | 475 |