Intra-day (n = 6)

Inter-day (n = 6)

Analytes

Conc. spiked (ng/mL)

Conc. measured (mean ± SD) (µg/mL)

Precision (%)

Accuracy

(%)

Conc. measured (mean ± SD) (µg/mL)

Precision (%)

Accuracy

(%)

Diclofenac

5

4.3 ± 0.6

13.2

86.7

4.9 ± 0.8

16.9

98.6

10

8.6 ± 0.7

8.2

86.2

9.3 ± 1.0

11.0

93.0

50

51.2 ± 6.0

11.7

102.4

48.2 ± 6.4

13.4

96.4

150

139.7 ± 23.0

16.5

93.1

130.5 ± 18.3

14.1

87.0

Fenoprofen

5

5.9 ± 0.2

4.1

117.0

4.7 ± 0.9

19.8

94.2

10

9.8 ± 0.6

6.0

98.1

9.4 ± 0.4

4.7

93.7

50

52.8 ± 3.2

6.1

105.6

47.9 ± 4.5

9.4

95.8

150

146.4 ± 14.3

9.8

97.6

133.2 ± 8.7

6.6

88.8

Flufenamic acid

5

4.4 ± 0.9

21.1

87.1

4.4 ± 1.1

24.7

88.5

10

5.5 ± 1.3

24.5

54.6

8.1 ± 2.5

30.4

80.9

50

43.0 ± 7.5

17.5

85.9

46.9 ± 8.7

18.5

93.8

150

102.7 ± 40.0

38.9

68.5

112.8 ± 37.0

32.8

75.2

Flunixin

5

5.0 ± 0.3

6.4

100.5

5.2 ± 0.6

11.0

104.7

10

10.3±0.9

9.0

103.4

11.0 ± 1.0

9.3

110.2

50

52.4±2.4

4.7

104.9

52.5 ± 4.2

8.1

104.9

150

145.9±15.0

10.3

97.3

142.7 ± 13.3

9.4

95.1

Indomethacin

5

4.5 ± 0.6

13.6

90.2

4.8 ± 0.8

16.9

95.9

10

8.5 ± 0.6

7.2

84.8

8.9 ± 0.4

4.8

88.7

50

49.0 ± 5.4

11.1

98.0

47.6 ± 7.8

16.3

95.3

150

130.1 ± 20.3

15.6

86.8

129.5 ± 21.5

16.6

86.4

Indoprofen

5

5.1 ± 0.4

7.1

101.1

5.1 ± 0.2

4.5

101.9

10

10.3 ± 0.5

4.8

102.6

10.4 ± 0.9

8.4

103.8

50

50.6 ± 2.4

4.8

101.2

53.1 ± 5.0

9.5

106.2

150

146.1 ± 7.9

5.4

97.4

143.9 ± 9.2

6.4

95.9

Ketoprofen

5

5.2 ± 1.4

27.3

103.3

5.1 ± 0.8

14.9

102.1

10

9.9 ± 1.0

10.2

99.2

10.1 ± 0.9

8.4

101.4

50

50.5 ± 2.0

4.0

101.1

50.0 ± 3.7

7.3

100.0

150

140.6 ± 9.1

6.5

93.7

141.2 ± 11.7

8.3

94.2

Ketorolac

5

4.6 ± 0.2

3.9

92.3

5.0 ± 0.7

13.6

100.5

10

9.8 ± 0.7

7.2

98.0

10.0 ± 0.4

4.4

99.7

50

46.4 ± 4.9

10.5

92.8

47.3 ± 5.8

12.3

94.5

150

139.8 ± 4.4

3.1

93.2

146.8 ± 12.5

8.5

97.9