| Analytes | Retention time | [M - H]− (m/z) | Ion transition (m/z) for screening and quantification | Ion transition (m/z) for confirmation |
| Diclofenac | 4.1 | 294 | 294 → 250 | 294 → 250, 214, 294 |
| Fenoprofen | 3.8 | 241 | 241 → 197 | 241 → 197, 241, 93 |
| Flufenamic acid | 4.6 | 280 | 280 → 215 | 280 → 215, 236, 176 |
| Flunixin | 4.4 | 295 | 295 → 251 | 295 → 251, 211, 209 |
| Indomethacin | 4.1 | 356 | 356 → 312 | 356 → 312, 297, 282 |
| Indoprofen | 3.3 | 280 | 280 → 236 | 282a → 236, 218, 77 |
| Ketoprofen | 3.5 | 253 | 253 → 209 | 255a → 209, 105, 194 |
| Ketorolac | 3.3 | 254 | 254 → 210 | 256a → 105, 77, 51 |
| Meclofenamic acid | 4.4 | 294 | 294 → 258 | 294 → 258, 214, 294 |
| Mefenamic acid | 4.4 | 240 | 240 → 196 | 240 → 196, 92, 240 |
| 6-MNA | 3.4 | 215 | 215 → 156 | 215 → 156, 215, 171 |
| Naproxen | 3.5 | 229 | 229 → 170 | 229 → 170, 169, 141 |
| Oxyphenbutazone | 3.5 | 323 | 323 → 295 | 323 → 295, 204, 134 |
| Phenylbutazone | 3.8 | 307 | 307 → 279 | 307 → 279, 188, 131 |
| Tolmetin | 3.5 | 256 | 256 → 212 | 258a → 119,91, 65 |
| Zomepirac | 3.7 | 290 | 290 → 245 | 292a → 139, 111, 108 |
| D9-phenylbutazone (IS-1) | 3.8 | 316 | 316 → 288 | 316 → 288 |
| Clonixin (IS-2) | 4.0 | 261 | 261 → 217 | 261 → 217 |