Time (min.) | A% | B% | Flow rate (µL/min.) |
0.0 | 90.0 | 10.0 | 100 |
2.0 | 50.0 | 50.0 | 200 |
5.0 | 50.0 | 50.0 | 200 |
5.5 | 20.0 | 80.0 | 200 |
7.5 | 20.0 | 80.0 | 200 |
8.0 | 90.0 | 10.0 | 200 |
9.0 | 90.0 | 10.0 | 200 |