| Steps | Temperature | Duration |
| Primary Denaturation | 95 c | 10 min |
|
| 95 c | 30 s |
| Secondary Denaturation | 65 c | 30 s |
| Annealing | 72 c | 30 s |
| Extention | 72 c | 7 min |
| Final Extention | Temperature | Duration |