| Micro-tube components | Concentration | Volume |
| PCR Master Mix | - | 10 µl |
| Distilled water | - | 1.8 µl |
| DNA template | - | 3 µl |
| DMSO | - | 1.6 µl |
| FO Primer | 10 pmol/µl | 1 µl |
| RO Primer | 10 pmol/µl | 1 µl |
| FI-I Primer | 10 pmol/µl | 0.8 µl |
| RI-I Primer | 10 pmol/µl | 0.8 µl |
| FI-II Primer | 10 pmol/µl | 0.8 µl |
| RI-II Primer | 10 pmol/µl | 0.8 µl |
| Micro-tube components | Total Volume | 20 µl |