Micro-tube components

Concentration

Volume

PCR Master Mix

-

10 µl

Distilled water

-

1.8 µl

DNA template

-

3 µl

DMSO

-

1.6 µl

FO Primer

10 pmol/µl

1 µl

RO Primer

10 pmol/µl

1 µl

FI-I Primer

10 pmol/µl

0.8 µl

RI-I Primer

10 pmol/µl

0.8 µl

FI-II Primer

10 pmol/µl

0.8 µl

RI-II Primer

10 pmol/µl

0.8 µl

Micro-tube components

Total Volume

20 µl