Method of fractioning | Caseins | Theoretical yield, % | Protein, % | Time of fractioning, h | Cost (in $) |
Gel-filtration on Sephadex (G-100, G-150) | κ | ~70 | 70 - 77 | 7 | 10000 - 15000 (dependence on system of liquid chromatography) |
αS1 and β | Repeated gel filtration ~30 - 40 | 75 - 80 | 14 | ||
Ion-exchanges chromatography on DEAE cellulose | αS1 | ~70 - 80 | >77 | 9 | 10000 - 15000 (dependence on system of liquid chromatography), |
β | >80 | ||||
κ | >75 | ||||
Differential precipitation in the presence of urea | αS1 | ~40 - 50 | >75 | 48 | ~1200 (with additional centrifuge for very big volumes In 1 - 3 L) |
β | >80 | ||||
Preparative electrophoresis in anode system of homogenous PAAG | αS1 | ~75 - 85 | >93 | 5 | 1500 (device prepared in laboratory of author) |
αS2 | >90 | ||||
β | >95 | ||||
κ | >90 |