Method of fractioning

Caseins

Theoretical yield, %

Protein, %

Time of fractioning, h

Cost (in $)

Gel-filtration on Sephadex

(G-100, G-150)

κ

~70

70 - 77

7

10000 - 15000

(dependence on system of liquid chromatography)

αS1 and β

Repeated

gel filtration

~30 - 40

75 - 80

14

Ion-exchanges chromatography on DEAE cellulose

αS1

~70 - 80

>77

9

10000 - 15000

(dependence on system of liquid chromatography),

β

>80

κ

>75

Differential precipitation in the presence of urea

αS1

~40 - 50

>75

48

~1200

(with additional centrifuge for very big volumes In 1 - 3 L)

β

>80

Preparative electrophoresis in anode system of homogenous PAAG

αS1

~75 - 85

>93

5

1500

(device prepared in laboratory of author)

αS2

>90

β

>95

κ

>90