| Culture cell | Naive | Primed |
| Embryonic tissue of origin | Mouse pre-implantation ICM | Mouse post implantation epiblast, Primate pre-implantation ICM |
| Cell line | Rodent ESC | hESC, rodent EpiSC |
| Pluripotency in vitro | Yes | Yes |
| Teratomas | Yes | Yes |
| Colony morphology | Domed | Flattened |
| Rapid self-renewal | Yes | No |
| Slow self-renewal | No | Yes |
| Response to LIF/BMP4 | Self-renewal | None |
| Response to FGF2/ERK | differentiation | None |
| Chimera formation | Yes | No |
| Pluripotency markers | Oct4, Nanog, Sox2, Klf2, Klf4 | Oct4, Sox2 ,Nanog |
| Single cell dissociation | Yes | No |
| Clonigenecity | High | Low |
| X-inactivation | No | Yes |
| Response to 2i | Self-renewal | Cell death |