| Gene | Primer name | Primer Sequence | Product length |
| SpeA | SpeA-F | 5'-CCAAGCCAACTTCACAGATC3' | 309 bp |
| SpeA-R | 5'-CCCTTCATGATTTGTTACCCC3' | ||
| SpeB | SpeB-F | 5'-GTGGAGTCTCTGACGGCTTC3' | 191 bp |
|
| SpeB-R | 5'-GTGTTTTCGGCACAAAAGGT3' |
|
| SPY1258 | SPY1258-F | 5'-AAAGACCGCCTTAACCACCT3' | 407 bp |
|
| SPY1258-R | 5'-TGCCAAGGTAAACTTCTAAAGCA3' |
|