Plant Growth regulator (mg/L)

Percentage of callus produced shoot

(%)

Number of shoot per clump green callus

(3 gram)

NAA

2,4-D

BAP

-

-

0

30 ± 4.5

11 ± 1.1

-

0.5

70 ± 8.7

27 ± 3.4

-

1.0

80 ± 9.6

31 ± 6.1

-

3.0

50 ± 3.4

17 ± 2.3

-

5.0

35 ± 5.7

12 ± 2.1

0.5

-

0

10 ± 1.3

7 ± 0.9

-

0.5

35 ± 3.4

18 ± 4.5

-

1.0

45 ± 6.6

19 ± 1.3

-

3.0

30 ± 3.2

18 ± 4.5

-

5.0

15 ± 3.1

6 ± 1.0

1.0

-

0

10 ± 2.1

15 ± 2.2

-

0.5

35 ± 6.7

18 ± 4.1

-

1.0

35 ± 8.1

15 ± 2.1

-

3.0

25 ± 2.3

15 ± 2.4

-

5.0

0

0

-

-

-

0.5

0

0

0

0.5

20 ± 2.1

9 ± 0.9

1.0

15 ± 2.3

12 ± 2.1

3.0

0

0

5.0

5 ± 1.5

8 ± 0.5

-

-

-

0

5 ± 0.9

0

0.5

15 ± 0.9

8 ± 0.5

1.0

1.0

15 ± 1.3

11 ± 1.3

3.0

10 ± 1.2

0

5.0

0

0