Plant Growth regulator (mg/L) | Percentage of callus produced shoot (%) | Number of shoot per clump green callus (3 gram) | ||
NAA | 2,4-D | BAP | ||
- | - | 0 | 30 ± 4.5 | 11 ± 1.1 |
- | 0.5 | 70 ± 8.7 | 27 ± 3.4 | |
- | 1.0 | 80 ± 9.6 | 31 ± 6.1 | |
- | 3.0 | 50 ± 3.4 | 17 ± 2.3 | |
- | 5.0 | 35 ± 5.7 | 12 ± 2.1 | |
0.5
| - | 0 | 10 ± 1.3 | 7 ± 0.9 |
- | 0.5 | 35 ± 3.4 | 18 ± 4.5 | |
- | 1.0 | 45 ± 6.6 | 19 ± 1.3 | |
- | 3.0 | 30 ± 3.2 | 18 ± 4.5 | |
- | 5.0 | 15 ± 3.1 | 6 ± 1.0 | |
1.0
| - | 0 | 10 ± 2.1 | 15 ± 2.2 |
- | 0.5 | 35 ± 6.7 | 18 ± 4.1 | |
- | 1.0 | 35 ± 8.1 | 15 ± 2.1 | |
- | 3.0 | 25 ± 2.3 | 15 ± 2.4 | |
- | 5.0 | 0 | 0 | |
- - - | 0.5 | 0 | 0 | 0 |
0.5 | 20 ± 2.1 | 9 ± 0.9 | ||
1.0 | 15 ± 2.3 | 12 ± 2.1 | ||
3.0 | 0 | 0 | ||
5.0 | 5 ± 1.5 | 8 ± 0.5 | ||
- - - |
| 0 | 5 ± 0.9 | 0 |
| 0.5 | 15 ± 0.9 | 8 ± 0.5 | |
1.0 | 1.0 | 15 ± 1.3 | 11 ± 1.3 | |
| 3.0 | 10 ± 1.2 | 0 | |
| 5.0 | 0 | 0 |