| Plant growth regulator (mg/L) | Percentage of explants induce callus (%) | Weight of green callus after second sub-culture | Colour of callus | |
| NAA | 2,4-D | |||
| 0 | 0 | 0 | - | - |
|
| 0.5 | 25 ± 4.1 | 0.16 ± 0.01 | Yellow-green |
|
| 1.0 | 30 ± 2.2 | 0.33 ± 0.12 | Yellow-green |
|
| 3.0 | 15 ± 3.3 | 2.11 ± 0.11 | Yellow-green |
|
| 5.0 | 10 ± 0.9 | 0.97 ± 0.23 | Yellow-green |
| 1.0 | 0 | 0 | - | - |
|
| 0.5 | 10 ± 1.1 | 0.34 ± 0.04 | Yellow-green |
|
| 1.0 | 10 ± 1.4 | 0.56 ± 0.06 | Yellow-green |
|
| 3.0 | 54 ± 4.5 | 4.98 ± 0.87 | Yellow-green |
|
| 5.0 | 34 ± 3.1 | 2.12 ± 0.56 | Yellow-green |
| 3.0 | 0 | 0 | - | - |
|
| 0.5 | 0 | 0.45 ± 0.05 | Yellow-green |
|
| 1.0 | 45 ± 6.7 | 0.97 ± 0.12 | Yellow-green |
|
| 3.0 | 35 ± 2.3 | 7.81 ± 1.23 | Dark-green |
|
| 5.0 | 30 ± 1.1 | 2.34 ± 0.45 | Yellow-green |
| 5.0 | 0 | 0 | - | - |
|
| 0.5 | 12 ± 2.1 | 4.56 ± 0.89 | Dark-green |
|
| 1.0 | 65 ± 7.2 | 5.49 ± 1.21 | Dark-green |
|
| 3.0 | 50 ± 6.7 | 6.45 ± 1.24 | Dark-green |
|
| 5.0 | 20 ± 2.3 | 1.89 ± 0.40 | Necrotic-green |
| 10.0 | 0 | 0 | - | - |
|
| 0.5 | 34 ± 3.3 | 7.50 ± 2.41 | Dark-green |
|
| 1.0 | 75 ± 9.5 | 8.45 ± 0.98 | Dark-green |
|
| 3.0 | 55 ± 3.5 | 6.54 ± 0.89 | Necrotic-green |