| P450 | Substrate | Final well [Substrate] µM | Volume of P450s µL per well | Volume of 100 mM K phosphate µL |
| CYP1A1 | EROD | 5 | 1 | 49 |
| CYP1A2 | CEC | 15 | 1 | 49 |
| CYP1B1 | EROD | 5 | 1 | 49 |