1. Carbon sources | Carbon sources | Glycerol | Soybean oil | Amidulin | Sucrose | Maltose | Tween-80 | Glucose |
PG (mg/L) | 12.79 | 9.49 | 10.71 | 12.71 | 16.63 | 11.37 | 7.34 | |
2. Maltose | concentration (g/L) | 2 | 4 | 6 | 8 | 10 | 12 |
|
PG (mg/L) | 11.097 | 16.14 | 25.01 | 23.09 | 22.93 | 23.20 |
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3. Nitrogen sources | Nitrogen sources | Ammonium sulfate | Ammonium oxalate | Peptone | Gelatin | Bean flour |
|
|
PG (mg/L) | 0.81 | 0.95 | 23.77 | 0.91 | 1.08 |
|
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4. Peptone | concentration (g/L) | 4 | 8 | 12 | 16 | 20 | 24 |
|
PG (mg/L) | 15.51 | 29.17 | 34.83 | 26.73 | 13.71 | 3.32 |
| |
5. NaCl | concentration (g/L) | 0 | 1 | 2 | 3 | 4 | 5 |
|
PG (mg/L) | 23.24 | 33.95 | 32.82 | 33.77 | 33.13 | 26.65 |
| |
6. KCl | concentration (g/L) | 0 | 1 | 2 | 3 | 4 | 5 |
|
PG (mg/L) | 27.61 | 28.64 | 33.59 | 33.24 | 32.76 | 22.09 |
| |
7. Tween-80 | concentration (%) | 0 | 1 | 2 | 3 | 4 | 5 |
|
PG (mg/L) | 33.79 | 36.74 | 35.91 | 34.09 | 33.39 | 31.65 |
| |
8. pH of medium | pH value | 4 | 5 | 6 | 7 | 8 | 9 |
|
PG (mg/L) | 0.01 | 28.13 | 32.51 | 38.20 | 40.67 | 36.45 |
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9. Inoculum size | Inoculum size (%) | 0.50 | 1.00 | 1.50 | 2.00 |
|
|
|
PG (mg/L) | 20.67 | 39.68 | 41.70 | 41.89 |
|
|
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10. Volume of medium | Volume (mL) | 25 | 50 | 75 | 100 | 125 | 150 |
|
PG (mg/L) | 47.57 | 47.10 | 41.89 | 40.87 | 30.18 | 17.87 |
| |
11. Rotational rate | Rotational rate (rpm) | 100 | 130 | 160 | 190 | 220 |
|
|
PG (mg/L) | 29.43 | 35.35 | 47.14 | 49.03 | 49.43 |
|
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12. Cultivation temperature | Temperature (˚C) | 26 | 28 | 30 | 32 | 34 |
|
|
PG (mg/L) | 24.14 | 47.45 | 52.23 | 53.68 | 36.77 |
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