1. Carbon sources

Carbon sources

Glycerol

Soybean oil

Amidulin

Sucrose

Maltose

Tween-80

Glucose

PG (mg/L)

12.79

9.49

10.71

12.71

16.63

11.37

7.34

2. Maltose

concentration (g/L)

2

4

6

8

10

12

PG (mg/L)

11.097

16.14

25.01

23.09

22.93

23.20

3. Nitrogen sources

Nitrogen sources

Ammonium sulfate

Ammonium oxalate

Peptone

Gelatin

Bean flour

PG (mg/L)

0.81

0.95

23.77

0.91

1.08

4. Peptone

concentration (g/L)

4

8

12

16

20

24

PG (mg/L)

15.51

29.17

34.83

26.73

13.71

3.32

5. NaCl

concentration (g/L)

0

1

2

3

4

5

PG (mg/L)

23.24

33.95

32.82

33.77

33.13

26.65

6. KCl

concentration (g/L)

0

1

2

3

4

5

PG (mg/L)

27.61

28.64

33.59

33.24

32.76

22.09

7. Tween-80

concentration (%)

0

1

2

3

4

5

PG (mg/L)

33.79

36.74

35.91

34.09

33.39

31.65

8. pH of medium

pH value

4

5

6

7

8

9

PG (mg/L)

0.01

28.13

32.51

38.20

40.67

36.45

9. Inoculum size

Inoculum size (%)

0.50

1.00

1.50

2.00

PG (mg/L)

20.67

39.68

41.70

41.89

10. Volume of medium

Volume (mL)

25

50

75

100

125

150

PG (mg/L)

47.57

47.10

41.89

40.87

30.18

17.87

11. Rotational rate

Rotational rate (rpm)

100

130

160

190

220

PG (mg/L)

29.43

35.35

47.14

49.03

49.43

12. Cultivation temperature

Temperature (˚C)

26

28

30

32

34

PG (mg/L)

24.14

47.45

52.23

53.68

36.77