Pre-treatment

Treatment

Post-treatment

SEM

P

12 h

24 h

36 h

48 h

Period

Animal

Plasma glucose, mmol/L

2.83

2.90

-

-

-

-

0.25

0.87

0.57

Plasma insulin, μ IU/ml

15.26 ^A

67.60^B

-

-

-

-

2.02

<0.01

0.10

Milk yield, g/12 h

263.19

273.57

253.03

265.10

282.03

273.30

17.54

0.47

<0.01

Milk fat, %

3.99 ^A

2.58^D

2.72^CD

3.20^BCD

3.33^ABC

3.467^AB

0.36

<0.01

0.15

g/12 h

10.55 ^A

7.00 ^C

6.92 ^C

8.46^BC

9.19^AB

9.44^AB

0.87

<0.01

<0.01

Milk protein, %

3.86 ^A

3.27^B

3.28^B

3.40^B

3.47^B

3.48^B

0.13

<0.01

<0.001

g/12 h

10.15 ^a

8.97^ab

8.33^b

9.09^ab

9.76^ab

9.51^ab

0.74

0.13

<0.01

Lactose %

4.20

4.09

4.27

4.31

4.28

4.25

0.21

0.83

0.06

g/12 h

11.07

11.14

10.80

11.46

12.04

11.64

0.97

0.68

<0.01

SNF, %

9.16

8.51

8.69

8.85

8.90

8.94

0.29

0.22

<0.001

g/12 h

24.10

23.23

22.01

23.57

25.01

24.46

1.84

0.47

<0.01