Sample after purification step | Total protein used per reaction (µg) | Total activity (pmol) | Specific activity (pmol/mg) | Fold purification | Yield (%) |
Cell free extract | 100 | 6.25 | 62.5 | 1 | 100 |
Ni-NTA affinity chromatography | 2.5 | 0.56 | 224 | 3.58 | 86.8 |
Superdex 200 gel filtration chromatography | 2.5 | 1.88 | 752 | 12.03 | 38.2 |