Sample after purification step

Total protein used per reaction (µg)

Total activity (pmol)

Specific activity

(pmol/mg)

Fold purification

Yield

(%)

Cell free extract

100

6.25

62.5

1

100

Ni-NTA affinity chromatography

2.5

0.56

224

3.58

86.8

Superdex 200 gel filtration chromatography

2.5

1.88

752

12.03

38.2